365 papers
This study characterizes the in vitro cleavage requirements and specificities of mycobacterial RNase E, revealing its dependence on a minimum substrate length of ~27 nucleotides, a preference for 5' monophosphates, sequence- and distance-dependent cleavage patterns, and potential product inhibition, while validating *M. smegmatis* as a suitable model for studying *M. tuberculosis* RNA degradation.
This study demonstrates that while the eIF4G-PABP closed-loop architecture is dispensable for productive translation initiation, it can be actively co-opted through specific proteolytic cleavage to form a dead-end complex that potently represses translation, thereby explaining the distinct translational outcomes of factor depletion versus viral cleavage.
By integrating multi-platform transcriptomics and membrane-aware proteomics, this study establishes a quantitative hierarchy of TRP channel expression in the adult mouse cortex, revealing that TRPML, TRPC, and TRPM subfamilies dominate while TRPA1 and TRPV1 remain below reliable detection thresholds.
This study demonstrates that implementing upstream strategies such as Cre/LoxP recombination and caspase inhibition effectively eliminates plasmid backbone and host cell DNA impurities in rAAV manufacturing, thereby significantly enhancing vector purity and safety for clinical applications.
The authors have withdrawn their manuscript because additional controls revealed that their initial conclusion regarding the Lsm1-7 complex's role in NMD was based on incorrect assumptions, rendering the RNA sequencing, phenotype, and RT-qPCR results invalid while other findings remain sound.
This study demonstrates that the host histone chaperone HIRA, through its deposition of the non-canonical histone variant H3.3 onto KSHV episomes, plays a critical and specific role in regulating viral latency and maintaining the stability of the viral genome, distinct from the function of the DAXX chaperone.
This study identifies apolipoprotein C1 (ApoC1) as a key host protein associated with hepatitis B virus particles that facilitates viral production and entry by modulating intracellular cholesterol levels and interacting with the HDL receptor SR-B1.
This study demonstrates that WAVE2 and REST/NRSF regulate the expression of clustered genes, such as protocadherins and beta-globin, by maintaining heterochromatin organization through the deposition of H3K9me3 and the prevention of aberrant CTCF/cohesin accumulation.
By comparing the mitochondrial proteomes of *Arabidopsis thaliana* and *Silene conica*, this study demonstrates that extensive mitochondrial gene loss drives significant remodeling of organellar translation machinery, including the retargeting of aminoacyl-tRNA synthetases, replacement of ribosomal subunits, and loss of the GatCAB complex.
This study identifies the transcriptional repressor Tex15 as a critical regulator of vomeronasal sensory neuron diversity, demonstrating that its absence disrupts the expression of specific pheromone receptors and abolishes male-male aggression behaviors in mice.
Celastrol alleviates SGLT2 inhibitor-induced diabetic hyperketonemia by suppressing hepatic ketogenesis through a PPAR-dependent mechanism that downregulates HMGCS2 expression and modulates free fatty acid levels.
The authors developed a cost-effective, high-resolution Tm-guided exon-exon junction RT-PCR method that ensures variant-specific detection of RNA splice isoforms lacking distinguishable exonic regions by designing primers that enforce junction-dependent amplification through precise melting temperature constraints.
This study establishes the first validated set of stable reference genes (specifically ACTB, EF1, RPL5, and 18SrRNA) for *Gryllodes sigillatus* by evaluating six candidates across multiple tissues using rigorous statistical methods, thereby enabling reliable RT-qPCR analyses for disease diagnosis and health monitoring in edible insect farming.
This study reveals that the long non-coding RNAs Airn and Kcnq1ot1 share distinct protein association patterns mirroring their repressive functions and identifies HNRNPU as an essential factor required for recruiting Polycomb Repressive Complexes to mediate long-range gene silencing, independent of its role in localizing these RNAs to chromatin.
This study demonstrates that chronic oxidative stress upregulates PTBP1 via CTCF-mediated promoter binding, which acts as a molecular switch to repress neuronal identity genes and activate senescence pathways, thereby driving neuronal dysfunction and aging.
This study demonstrates that splice-switching antisense oligonucleotides targeting exon 2 of the H3-3A gene effectively induce exon skipping to silence the H3.3K27M onco-histone, restore global H3K27me3 marks, and extend survival in mouse models of H3.3K27M-altered diffuse midline glioma.
This study reveals that nuclear rupture in LMNA cardiomyopathy drives disease progression by causing the loss of RNA polymerase II and subsequent global transcriptional deficiency, a process that is partially mitigated but ultimately overwhelmed by the rapid re-rupture of nuclei despite the cardioprotective action of ESCRT-III-mediated resealing.
This study reports the discovery and near-atomic resolution structure of the CAGE complex, a conserved, ~1 MDa hollow protein assembly found across diverse prokaryotic and eukaryotic microbes, suggesting an ancient evolutionary origin and potential roles in cellular transport or homeostasis.
This study reveals that in *Bacillus subtilis*, EPE toxin production is post-transcriptionally coordinated by the RNA-binding proteins Kre and SpoVG, which link the competence regulator ComK to the cannibalism pathway by repressing and activating *epeX* expression, respectively.
This study demonstrates that in genetically identical armadillo quadruplets, persistent transcriptomic individuality arises from stable differences in monocyte lineage expansion and inflammatory gene programs, linking early stochastic events to lasting functional divergence in immune cell composition.